The Little Foxes (1941)
The line was spoken by Alexandra Giddens (Teresa Wright) after her evil Southern aristocratic mother Regina Giddens (Bette Davis) asked her: "Would you like to talk with me, Alexandra? Would you - like to sleep in my room tonight?"
you guys give up, or are you thirsty for more
Fact: The line “you guys give up, or are you thirsty for more?” was improvised by Macaulay Culkin, and it is now one of the most popular pieces of dialogue from the film.