In the course of which hostilities did Rome suffer what is seen as its greatest defeat?

Question

In 1527 Rome was comprehensively sacked and its people massacred (although not by an invading country but by troops of discontented foreign soldiers) in the course of what war?

Answer 1

The second Punic War
The war was one of three campaigns fought between Rome and Carthage over the period 264 BCE to 146 BCE. The battle was the Battle of Cannae fought in 216 BCE in Apulia, southeast Italy. The Carthaginians and their allies, led by Hannibal, surrounded and in one day practically annihilated the much larger Roman and Italian army. Deaths on the Roman and allied side are given by some Roman writers at the time as between 70,000 and over 90,000. Deaths among Hannibal's forces are thought to have been 8,000 to 10,000.